Journal: Medicine

Article Title: Immune checkpoint proteins PD-1 and TIM-3 are both highly expressed in liver tissues and correlate with their gene polymorphisms in patients with HBV-related hepatocellular carcinoma

doi: 10.1097/MD.0000000000005749

Figure Lengend Snippet: PD-1 expression in liver tissues (×200). (A) Tumor tissues of HCC; (B) tumor adjacent tissues of HCC; (C) liver tissues of cirrhosis; (D) IHC score of PD-1 expression in (a) tumor tissues of HCC, (b) tumor adjacent tissues of HCC, and (c) liver tissues of cirrhosis. Black arrows indicate examples of positive staining cells. HCC = hepatocellular carcinoma, IHC = immunohistochemistry, PD-1 = programmed death-1.

Article Snippet: The sections were incubated with primary antibodies mouse monoclonal anti-PD-1 antibody (Abcam, Cambridge, MA) at a dilution of 1:50 or rabbit polyclonal anti-TIM-3 antibody (BioVision, Milpitas, CA) at a dilution of 1:150 at 37 °C for 30 minutes.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Medicine

Article Title: Immune checkpoint proteins PD-1 and TIM-3 are both highly expressed in liver tissues and correlate with their gene polymorphisms in patients with HBV-related hepatocellular carcinoma

doi: 10.1097/MD.0000000000005749

Figure Lengend Snippet: PD-1 expression in tumor tissues of HBV-related HCC (n = 171), tumor adjacent tissues of HCC (AT, n = 171), and cirrhotic liver tissues (LC, n = 34) according to the genotypes of PD1 polymorphism. AA, AG, and GG = genotype AA, genotype AG, and genotype GG of PD1 +8669 G/A (rs10204525) polymorphism, HBV = hepatitis B virus, HCC = hepatocellular carcinoma, IHC = immunohistochemistry, AT = adjacent tissues of HCC, LC = liver cirrhosis, PD-1 = programmed death-1.

Article Snippet: The sections were incubated with primary antibodies mouse monoclonal anti-PD-1 antibody (Abcam, Cambridge, MA) at a dilution of 1:50 or rabbit polyclonal anti-TIM-3 antibody (BioVision, Milpitas, CA) at a dilution of 1:150 at 37 °C for 30 minutes.

Techniques: Expressing, Immunohistochemistry

Journal: Heliyon

Article Title: Combining immunotherapy with high-dose radiation therapy (HDRT) significantly inhibits tumor growth in a syngeneic mouse model of high-risk neuroblastoma

doi: 10.1016/j.heliyon.2023.e17399

Figure Lengend Snippet: HDRT inhibits tumor growth at early time point. (A) Schema of anti-PD1 antibody and radiation treatments in 9464D syngeneic intrarenal tumors. Mice were enrolled on day 0. Mice were treated with anti-PD1 antibody on day 0, 3, 6 and 8 Gy fractions on day 3, 5 and 7 post-enrollment. Mice were subjected to early sacrifice (early time point) on day 9 and late sacrifice at 1.5 cm 3 tumor size. (B) Representative gross images of tumors for each treatment group at early sacrifice time point. Mice were sacrificed on Day 9 as described in A. (C) Tumor volume at early time point (Day 9). Tumor volume was measured by ultrasound. (D) Tumor weight at early time point (Day 9).

Article Snippet: Mice received 200 μg of anti-PD1 antibody (CD279, BioX Cell, clone 29F.1A12) [ , , ] via intraperitoneal injections on day 0, 3 and 6 post-enrollment.

Techniques:

Journal: Heliyon

Article Title: Combining immunotherapy with high-dose radiation therapy (HDRT) significantly inhibits tumor growth in a syngeneic mouse model of high-risk neuroblastoma

doi: 10.1016/j.heliyon.2023.e17399

Figure Lengend Snippet: Combination of anti-PD-1 blockade and HDRT inhibits 9464D tumor growth. (A) Tumor growth with time for 4 treatment groups. Tumor volume was measured by ultrasound. Mice were enrolled on day 0. Mice were treated with anti-PD1 antibody on day 0, 3, 6 and 8 Gy fractions on day 3, 5 and 7 post-enrollment. (B) Kaplan–Meier analysis of mice, bearing 9464D tumors, from 4 treatment groups. Survival was defined as the day when tumor volume reached 1.5 cm 3 and mouse was sacrificed.

Article Snippet: Mice received 200 μg of anti-PD1 antibody (CD279, BioX Cell, clone 29F.1A12) [ , , ] via intraperitoneal injections on day 0, 3 and 6 post-enrollment.

Techniques:

Journal: Journal of Inflammation Research

Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

doi: 10.2147/JIR.S347777

Figure Lengend Snippet: Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For flow cytometry, cell suspensions from human peripheral blood and tissue samples were stained with following antibodies cocktail at 4°C for 40 mins: Percp-Cy TM 5.5 mouse anti-human CD45 (564,105, Clone HI30, BD Pharmingen TM , San Diego, USA), BV510 mouse anti-human CD3 (564,713, Clone HIT3α, BD Pharmingen TM , San Diego, USA), APC-R700 mouse anti-human CD4 (564,975, Clone RPA-T4, BD Pharmingen TM , San Diego, USA), APC-Cy TM 7 mouse anti-human CD8 (557,834, Clone SK1, BD Pharmingen TM , San Diego, USA), PE mouse anti-human TCR γδ (555,717, Clone B1, BD Pharmingen TM , San Diego, USA), Alexa Fluor ® 647 mouse anti-human CD66b (561,645, Clone G10F5, BD Pharmingen TM , San Diego, USA), BV421 mouse anti-human PD-1 (564,323, Clone MIH4, BD Horizon TM , San Diego, USA), PE-Cy TM 7 mouse anti-human CD274 (558,017, Clone MIH1, BD Pharmingen TM , San Diego, USA).

Techniques: Expressing, Flow Cytometry